I work on two plant systems, chickpea, a legume and Arabidopsis, a model plant.Chickpea is the integral part of the daily diet of majority of the population. The yield of chickpea has not improved with time because of a number of stresses (both biotic and abiotic) severely affecting the productivity. Our group is trying to understand drought tolerance and wilt resistance in chickpea using the tools of functional genomics. We have generated thousands of expressed sequence tags (ESTs) by suppression subtraction hybridization (SSH) to identify differentially expressed genes in drought-tolerant/wilt resistant and drought susceptible/wilt susceptible genotypes in chickpea. Several genes like ERF, NAC, DREB etc. have been characterized in detail. Of late, microRNAs have emerged as major players in gene regulation. We have identified and characterized salt- and wilt-stress responsive microRNAs for the first time. We are also using mapping population(s) for identifying genes/QTL involved in drought tolerance and wilt resistance.
I also work in the area of Host delivered RNAi for nematode resistance. We have identified and cloned nematode-responsive root specific promoters, in Arabidopsis, as an alternative to constitutive promoters for expressing dsRNA to induce RNAi. We have observed 40-60 % reduction in nematode infection in transgenic plants expressing dsRNA for nematode genes. Our group has longstanding interest in isolating promoters with specific spatial and temporal expression patterns. Such promoters can be ultimately used for manipulating responses to biotic and abiotic stresses. Several tissue-specific promoters like root-, anther- and trichome-specific promoters have been isolated and characterized.
Awards & Honors
BOYSCAST Fellowship by DST for year 2003-04
- Kohli D, Joshi G, Deokar AA, Bhardwaj AR, Agarwal M, Katiyar-Agarwal S, Srinivasan R and Jain PK (2014)Identification and Characterization of Wilt and SaltStress-Responsive MicroRNAs in Chickpea throughHigh-Throughput Sequencing. PLoS ONE 9(10): e108851. doi:10.1371/journal.pone.0108851.
- Deokar AA, Kondawar V, Kohli D, Aslam M, Jain PK, Karuppayil M, Varshney RK and Srinivasan R (2014) The CarERF genes in chickpea ( Cicerarietinum L.) and the identification of CarERF116 as abiotic stress responsive transcription factor. Func. Int. Genomics DOI 10.1007/s10142-014-0399-7.
- Satheesh V, Jagannadham PTK, Chidambaranathan P, Jain PK and Srinivasan R (2014) NAC transcription factor genes: genome-wide identiﬁcation, phylogenetic, motif and cis-regulatory element analysis in pigeonpea (Cajanuscajan (L.) Millsp.) Mol. Biol. Rep. DOI 10.1007/s11033-014-3669-5.
- Khanna SM, Taxak PC, Jain PK, Saini R and Srinivasan R (2014) Glycolytic Enzyme Activities and Gene Expression in Cicerarietinum Exposed to Water-Deficit Stress. Appl.Biochem. Biotech. 173:2241-2253. DOI 10.1007/s12010-014-1028-6.
- Sharma R, Arya S, Patil SD, Sharma A, Jain PK, Navani, NK and Pathania R (2014) Identification of Novel Regulatory Small RNAs in Acinetobacterbaumannii. PLoS ONE 9(4):e93833. doi:10.1371/journal.pone.0093833.
- Khanna SM, Choudhary P, Saini R, Jain, PK and Saini R (2014) Specific activities and transcript levels of glycolytic enzymes under dehydration in chickpea (Cicerarietinum L.) seedlings. Legume Res.
- Khanna SM, Choudhary P, Saini R, Jain, PK and Srinivasan R (2014) Effect of water deficit stress on growth and physiological parameters in chickpea cultivars differing in drought tolerance. Annals Biol. 30:77-84.
- Choudhary P, Khanna SM, Jain PK, Bharadwaj C, Kumar J, Lakhera PC and Srinivasan R (2013) Molecular Characterization of Primary Gene Pool of Chickpea Based on ISSR Markers. Biochemical Genet. 51: 306-322.
- Kumar T, Bharadwaj C, Tara Satyavathi C and Jain PK (2013) A high throughput, improved rapid and reliable genomic DNA extraction protocol from chickpea (Cicer arietinum L.) Vegetos 26 (2): 185-190.
- Choudhary P, Khanna SM, Jain PK, Bharadwaj C, Kumar J, Lakhera PC and Srinivasan R (2012) Genetic structure and diversity analysis of primary gene pool of chickpea using SSR markers. Genet Mol. Res. 11:891-905.
- Gupta NC, Jain PK, Bhat SR and Srinivasan R (2012) Upstream sequence of fatty acyl-CoA reductase (FAR6) of Arabidopsis thaliana drives wound-inducible and stem-specific expression. Plant Cell Rep.31:839-850.
- Vajinder Kumar V, Thakare DR, Saha DN Jajoo A, Jain PK, Bhat SR and Srinivasan R. (2012) Characterization of upstream sequences of the peroxidase gene, Atprx18 of Arabidopsis thaliana. J. Plant Biochem. Biotech. 21(1):121–127.
- Singh, NK…… Jain PK. et al. (2012) The first draft of the pigeonpea genome sequence. J. Plant Biochem. Biotech. 21(1): 98–112.
- Deokar, AA Kondawar, V, Jain, PK, Karuppayil, SM, Raju, NL Vadez, V, Varshney, R and Srinivasan, (2011) Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress. BMC Plant Biology 11:70.
- Patil, G, Deokar AA, Jain, PK, Thengane, RJ and Srinivasan R, 2009. Development of a phosphomannose isomerase-based Agrobacterium-mediated transformation system for chickpea (Cicerarietinum L.) Plant Cell Reports 28:1669-1676.
- Patil, G, Patel, R, Jaat, R, Pattanayak, A, Jain, PK and Srinivasan, R. 2009 Glutamine improves shoot morphogenesis in chickpea (Cicerarietinum L.) Acta Physiol. Plant. 31:1077-1084
- Saha,D, Prasad, A, Sujatha, TP, Kumar, V, Jain, PK, Bhat, SR and Srinivasan R, 2006. In silico analysis of the Lateral Organ Junction (loj) gene and promoter of Arabidopsis thaliana.In Silico Biology 7, 0002. </isb/2006/07/0002/>
- Sunkar, R, Girke, T, Jain, PK, and Zhu, J-K. 2005 Cloning and characterization of microRNAs from rice. Plant Cell 17: 1397-411.