Isolation of plant genes and promoters
(PI-Anita Grover) | Identification of pathogen-inducible genes and promoters and using them for developing fungus (Alternaria)-resistant transgenic Brassica junceaplants (PI-Dr. Anita Grover) | - Identification and isolation of pathogen-inducible genes in B. juncea and development of fungus (Alternaria)-resistant transgenic B. juncea plants using few of these genes
- Isolation and characterization of pathogen-inducible strong promoters and their functional validation
- To study the response of B. juncea and its wild relative (Camelina sativa and Sinapis alba) and Erurapajunceato Alternaria infection and to salicylic acid and jasmonic acid treatment
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| | Cloning, validation and characterization of insecticidal genes from legumes in mustard (PI-Dr. RekhaKansal) | - Cloning of lectin and protease inhibitor genes under phloem specific promoter
- Generation of transgenic mustard for validation and evaluation of the efficacy of the genes
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| | Isolation and characterization of stress responsive genes and promoters in chickpea (PI-Dr. P. K. Jain) | - To identify and characterize genes and microRNAs associated with Fusarium wilt and drought stress in chickpea
- To identify and characterize drought and wilt stress-responsive promoters
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| | Pigeonpea viruses: characterization of viral genome, their genes and transgenic control (Dr. B. L. Patil) | - Next generation sequencing of small RNAs from virus/SMD (Sterility mosaic disease) infected Pigeonpea, their analysis, and annotation of PPSMV (Pigeonpea sterility mosaic virus) genome sequences
- Identification and characterization of viral genes and silencing suppressors
- Sequencing and characterization of PPSMV isolates across India
- RNAi-mediated genetically engineered resistance to PPSMV
- Developing VIGS vector using Geminivirus (MYMIV) infecting pigeonpea
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| | Cloning and characterization of genes involved in rice Xanthomonasoryzaepvoryzae interaction (PI-Dr. Rhitu Rai) | - Cloning and characterization of effectors from blight pathogen and identification of their host target genes in rice
- Identification of susceptibility genes in rice for targeted suppression
- Identification and fine mapping of new resistance (R) genes from Oryza germplasm
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| | Isolation and characterization of salt stress responsive gene(s) and promoter(s) in chickpea (PI-Ms. Nimmy M. S.) | - To identify, isolate and characterize genes associated with salinity tolerance in chickpea
- To isolate and characterize the promoters of the above identified salt responsive genes in chickpea
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Biotechnological approaches for increasing crop productivity
(PI-Dr. S. R. Bhat) | Generation and characterization of genetic stocks for heterosis breeding (PI-Dr. S. R. Bhat) | - Development and characterization of genetic stocks (CMS, restorer and haploid-inducer lines) for heterosis breeding in Brassica
- Molecular analysis of polyembryony in citrus/guggul
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| | Phytohormone modulation for yield enhancement in mustard (PI-Dr. P. K. Dash) | - Modulation of phytohormone homeostasis for enhancement of grain number in Brassica
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| | Genome editing in oilseed Brassica for crop improvement (PI-Dr. N. C. Gupta) | - Standardization of CRISPR/Cas system for gene editing in B. juncea
- Construction of CENH3-specific CRISPR/Cas cassettes and transformation of B. juncea for the production of CENH3 mutant lines for use in developing haploid inducer lines
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| | Enlarging genepool of Brassica junceathrough resynthesis and introgression from wild species (PI-Mr. Mahesh Rao) | - To introgressAlternariabrassicaeresistance gene(s) into mustard from the wild species Diplotaxiserucoidesand identify D. erucoidesILS through markers
- Cytogenetic study of hybrids and ILS to understand chromosomal behaviour
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| Genomics and molecular markers in crop plants (PI-Dr. N. K. Singh) | Functional genomics of mandate crops including rice, wheat, pigeonpea etc. for identification of genes/markers for grain quality and stress tolerance (PI-Dr. N. K. Singh) | - To organize infrastructure and develop team of scientists and carry out high throughput genotyping and expression studies to provide support to rice, pigeonpea and mango genomics
- To identify new genes and markers in riceforyield, quality and salt tolerance and wheat quality, drought and thermal tolerance
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| | Cloning and characterization of plant disease resistance genes/alleles and promoters using genomics and bioinformatics approaches (PI-Dr. T. R. Sharma) | - Allele mining for selected plant disease resistance genes from oryza spp.
- Cloning and characterization of pathogen inducible promoters and understanding host immunity
- Expression profiling of rice lines containing blast resistance genes
- Development of plant genome databases and comparative genome analysis among different crops
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| | Functional genomics of heterosis and temperature tolerance in pigeonpea (PI-Dr. Kishore Gaikwad) | - Characterization of genes responsible for cytoplasmic male sterility and fertility restoration in pigeonpea
- Mapping and characterization of genes responsible for thermotolerance in pigeonpea
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| | Salt tolerance in riceand pigeonpea (PI-Dr. Vandna Rai) | - To generate polymorphisms between the susceptible genotype HD2932 and the resistant genotype SW89-5422 and to evaluate the segregating populations of the biparental crosses between HD2932 and SW89-5422 for the leaf spot resistance.
- To map the loci underlying the leaf spot resistance in the biparental cross.
- To validate the mapped genes/QTLs for the resistance to leaf spot disease and to investigate the feasibility of using the above said genes and QTLs for subsequent MAS for varietal development.
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| | Phenotyping of rice, wheat and pigeonpea for evaluation and validation of yield contributing traits for targeted breeding (PI-Mr. R. S. Jaat) | - Identification of genes and markers for salt tolerance in cultivated and wild rice
- Identification of QTL and stress related proteins for salt tolerance in pigeonpea
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| | Mapping of QTLs for abiotic stress tolerance in rice (PI-Dr. S V A C R Mithra) | - To map QTLs and identify candidate genes for moisture stress tolerance in rice
- To validate the QTLs for their subsequent utility in rice breeding programmes
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| | Molecular cloning and characterization of panicle blast resistance genes in rice (PI-Dr. A. U. Solanke) | - Mapping of genes/QTLs for panicle blast resistance in rice
- Expression profiling of rice lines under panicle blast infestation
- Molecular cloning and characterization of selected panicle blast associated genes
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| | Expression analysis of small RNAs involved in the plant defence response in Rice - Rhizoctonia interaction (PI-Mr. RA Nagar) | - Sequencing of total small RNAs from sheath blight resistance and susceptible rice lines.
- Identification of differentially expressed small RNA in sheath blight resistance Vs susceptible rice lines.
- Cloning and functional validation of differentially expressed small RNAs and their target mRNAs
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Transgenic crops for biotic stress resistance
(PI-Dr. Sarvjeet Kaur ) | Isolation of insecticidal genes from native Bacillus thuringiensis isolates(PI-Dr. Sarvjeet Kaur) | - Identification of novel Cry and VIP genes from indigenous isolates of Bacillus thuringiensis
- Cloning and characterization of novel Cry and VIP genes
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| Management of gram pod borer by VIP and RNAi (PI-Dr. DebasisPattanayak) | - Construction of codon-modified Bt-VIP3Aa14
- Validation in planta (Tobacco and Pigeonpea)
- Design and validate RNAi-mediated resistance to Helicoverpa
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| Novel genes and strategies for aphid resistance in mustard (PI-Dr. R. C. Bhattacharya) | - Development of RNAi mediated genetic resistance in mustard cultivars against mustard aphids
- Identification of host-immunity genes and pathways to develop aphid resistance in mustard
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| Management of gram pod borer by Bt ICPs (PI-Dr.Rohini Sreevathsa) | - Genetic transformation of pigeonpea by Bt-Cry1Ac-F
- Genetic transformation of pigeonpea by Bt-Cry2Aa
- Molecular analysis and insect bioassays
- Gene stacked -pigeonpea (Cry1Ac-F+Cry2Aa)
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| Functional validation of novel insecticidal gene(s) cloned from Bacillus thuringiensis (PI-Dr. AumreetamDinabandhu) | - Development of plant transformation vector carrying novel VIP3Aa44 gene
- In planta validation of the genes and development of pigeonpeatransgenics
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| Adaptation of wheat to climate change induced abiotic stress (PI-Dr J. C. Padaria ) | Cloning and transfer of genes for high temperature tolerance in wheat (PI-Dr. J. C. Padaria) | - Isolation and characterization of high temperature stress responsive genes from thermotolerant plant systems
- Validation of the identified high temperature stress responsive genes in wheat
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| | Marker assisted introgression of gene(s)/QTLs for resistance to Bipolaris leaf spot (Bipolarissorokiniana) in wheat (PI-Dr. Sanjay Singh) | - Identification of donors, recipient, QTLs and validation of markers for resistance to Bipolaris leaf spot in wheat
- Transfer of genes/QTLs for Bipolaris leaf spot resistance in wheat using MAS
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| | Cloning and characterization of genes regulating grain quality under terminal heat stress in wheat (PI- Dr. SharmisthaBarthakur) | - To identify genes and regulatory elements involved during grain development and affecting end use quality of wheat flour under high temperature stress
- To functionally characterize and validate the selected genes
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| | Identification and cloning of gene(s) for salt tolerance in wheat (PI-Dr.Kanika) | - Evaluation of land races and wild relatives of wheat and microbes for tolerant to salinity
- Cloning and characterization of genes related to salt tolerance from the identified genotypes
- Validation of the selected genes in wheat
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| | Molecular mapping and cloning of gene(s) /QTLs for drought tolerance in wheat (PI-Dr. Monica Dalal) | - To identify wheat genotypes with contrasting RSA under drought and osmotic stress by phenotyping a diverse set of wheat genotypes
- Isolation and characterization of genes associated with root architecture under drought stress
- Validation of role of candidate wheat genes for drought tolerance in wheat/tobacco/Arabidopsis
- Mapping of QTL(s) for drought tolerance in wheat
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| Improvement of nutrient use efficiency in cereal crops (PI-Dr. P. K. Mandal) | Identification and characterization of gene(s) of nitrogen uptake in wheat(PI-Dr. Subodh Sinha) | - Evaluation of different wheat genotypes for nitrogen uptake
- Identification and characterization of gene(s) associated with nitrogen uptake in wheat
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| Identification and characterization of gene(s) of nitrogen metabolism in wheat(PI-Dr. P. K. Mandal) | - Selection of contrasting wheat genotypes for nitrogen use efficiency
- Studies of key enzymes involved in nitrogen use pathway in contrasting wheat genotypes
- Identification and cloning of differentially expressed genes in contrasting wheat genotypes
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