Illumina iScan for Infinum assay: This facility has both BeadXpress Reader and iScan system from Illumina Inc. for SNP genotyping. Both are robust platforms for genotyping applications.BeadXpress Reader has a dual colour detection system and is a low to medium throughput system. It works on veracode technology and is compatible with GoldenGate Genotyping (GGGT) assay. It can handle multiplexing in terms of SNPs from 48 to 1536. It terms of samples, the multiplexing capacity is to the tune of 8 to 96. In addition, gene expression and protein based assays can also be carried out using this system.iScan system uses high performance lasers, optics and detection systems to offer sub-micron resolution and as a result is useful for medium to high throughput applications. It has enhanced multiplexing ability to the tune of 12 million SNPs. Apart from genotyping, it can also be used for CNV analysis, DNA methylation and gene expression profiling.
For the readily available assays, any number of samples in multiples of 32 (up to 3000 SNPs) or 24 (up to 60000 SNPs) can be ordered depending on the sample size of the experiment. If the number of SNPs to be assayed is still more, sample size could be in multiples of 12. Please note that the minimum sample size required to be purchased from the manufacturer is 1152 when the user is developing his/her own assay. And customization charges are levied separately by the manufacturers. Good quality sample DNA and the consumables from the user are necessary prerequisites for carrying out the assay.
According to the number of samples, number of HD chip, the species to be genotyped and the requirement of each user, the cost of processing will be worked out for each experiment.
In-Charge: Dr Amitha Mithra
E mail Id: amitha[at]nrcpb[dot]org