ABI3730/ABI3500

Page Updated On : 22/02/2018 - 10:48:53
Printer-friendly version

ABI3500

Application: Sequencing

1. User instructions

  1. DNA should be of the following concentration:
    • PCR product: as such (15 µl)
    • Genomic or cDNA: 30 ng/ µl
    • Plasmid DNA: 200-300 ng/ µl
  2. Either Exo-SAP purified DNA or impure DNA can be sent. Purification charges are separate. It is preferable to send the template without purification since if the reaction fails because of improper purification, we will not be held responsible.
  3. Forward primer (FP)/ reverse primer (RP)/both the primers, as per need, should be properly labelled and sent for sequencing. If M13 universal primers need to be used, then the primers need not be sent. Please note that each reaction is counted separately. If PCR product has to be sequenced using both FP and RP, then it would be counted as two separate reactions and charged accordingly.
  4. Minimum number of samples: 24. Number of samples in multiples of 24 can also be sent. When the sample number is more than 96, please ask for ABI 3730xl processing as that would be cheaper.

 

2. Charges(in INR):

Item

ICAR

Non-ICAR

Sequencing with purification

 

10000 for 24 samples

11250 for 24 samples

Sequencing without purification

 

7000 for 24 samples

7500 for 24 samples

 

    Application: Genotyping

    1. User instructions

    1. 10-25 µl of PCR product should be supplied, as per need.
    2. If PCR products have been pooled it should be clearly mentioned. If no pooling is required, that should also be instructed.
    3. If pooling is done by the user himself/herself, information on how pooling was performed should be provided. While NED and PET dyes are used for pooling along with stringer dyes such as FAM and VIC, then the pooling should be in the following ratio:
      1. FAM: VIC:NED:PET ::  1:1:2:4
      2. Pooling can also be done by us at nominal charges.  
    4.  The details of the fluorescent dye and expected PCR product size needs to be furnished to choose the appropriate internal size standard while carrying out the capillary electrophoresis.
    5. Minimum number of samples: 24. Number of samples in multiples of 24 can also be sent. When the sample number is more than 96, please ask for ABI 3730xl processing as that would be cheaper.

     

    2. Charges(in INR):

    Item

    ICAR

    Non-ICAR

    Genotyping with pooling

     

    3250 for 24 samples

    3750 for 24 samples

    Genotyping without pooling

     

    2750 for 24 samples

    3250 for 24 samples

     

    ABI 3730xl

    Application: Sequencing

    1. User instructions

    1. DNA should be of the following concentration:
      • PCR product: as such (15 µl)
      • Genomic or cDNA: 30 ng/ µl
      • Plasmid DNA: 200-300 ng/ µl
    2. Either Exo-SAP purified DNA or impure DNA can be sent. Purification charges are separate. It is preferable to send the template without purification since if the reaction fails because of improper purification, we will not be held responsible.
    3. Forward primer (FP)/ reverse primer (RP)/both the primers, as per need, should be properly labelled and sent for sequencing. If M13 universal primers need to be used, then the primers need not be sent. Please note that each reaction is counted separately. If PCR product has to be sequenced using both FP and RP, then it would be counted as two separate reactions and charged accordingly.
    4. Minimum number of samples: 96 and multiples of 24 thereafter (120, 144 and so on)

     

    2. Charges(in INR):

    Item

    ICAR

    Non-ICAR

    Sequencing with purification

     

    38800 for 96 samples

    42500 for 96 samples

    Sequencing without purification

     

    27000 for 96 samples

    30000 for 96 samples

    Application: Genotyping

    1. User instructions

    1. 10-25 µl of PCR product should be supplied, as per need.
    2. If PCR products have been pooled it should be clearly mentioned. If no pooling is required, that should also be instructed.
    3. If pooling is done by the user himself/herself, information on how pooling was performed should be provided. While NED and PET dyes are used for pooling along with stronger dyes such as FAM and VIC, then the pooling should be in the following ratio:
      • FAM: VIC:NED:PET ::  1:1:2:4
      • Pooling can also be done by us at nominal charges.  
    4.  The details of the fluorescent dye and expected PCR product size needs to be furnished to choose the appropriate internal size standard while carrying out the capillary electrophoresis.
    5. Minimum number of samples: 96 and multiples of 24 thereafter (120, 144 and so on)

     

    2. Charges(in INR):

    Item

    ICAR

    Non-ICAR

    Genotyping with pooling

     

    11800 for 96 samples

    13250 for 96 samples

    Genotyping without pooling

     

    11000 for 96 samples

    12250 for 96 samples

    In-Charge: Dr Amitha Mithra

    E mail Id: amithanrcpborg